Česky Logo firmy AlphaBio EcoService, s.r.o
Introduction of the Company
Company Reference List
Case studies
Contact Information
AlphaBio EcoService, s.r.o.
Vlastina 23,161 00 Praha - 6
tel. +420-2-96 40 08 03, fax. +420-2-33 31 30 86
E-mail: Praha - abes@wo.cz Ostrava - suk.abes@worldonline.cz

Introduction of the Company


AlphaBio EcoService, Ltd. (ABES) is a certified ALPHA BIOREMEDIATION COMPANY, a proud member of a growing family of more than 120 companies built by ALPHA ENVIRONMENTAL BIOSYSTEMS, Inc., Lee's Summit, MO, whose scientists and researchers developed ALPHA bioprocesses, one of the prominent tools in use by ABES. Among the most renowned projects that elevated the ALPHA bioprocesses into accepted commercial tool in the U.S.A., Europe, South Africa and Japan and received universal public attention belong bioremediation of the famous MEGA BORG Supertanker Disaster Oil Sill in the Bay of Mexico, bioremediation of the major oil spill of and on the coast near Galveston, TS, activities in the Gulf during and after the Operation Desert Storm and .... of course, less visible, hundreds of successful jobs in bioremediation of hydrocarbon-contaminated soil, aquifer, groundwater, surface waters, oil and grease polluted lagoons or storage tanks. ALPHA processes have also enhanced operation of dozens of waste water treatment plants (WWTP) in the U.S., Republic of South Africa, U.K., Mexico, Denmark and the Czech Republic.

Abstract from Company Reference List


Enhancement of treatment process in municipal waste water treatment plants :


Enhancement of treatment process in industrial waste water treatment plants :
WWTP VETAS CHOTÝČANY (rendering plant)
WWTP LIKO NÝŘANY (yeast production)


Design and delivery of systems for biological treatment of oil and grease polluted cooling water:
KAVALIER Glassworks, Sázava (closed circuit system)


Treatment of surface water polluted by oil products:
Continuous flow treatment of over 350.000 m3 of lake water of Staroměstský lake in TELČ


Bioremediation of fats floating on a surface of a waste water reservoir:
UNILEVER - Van Den Bergh PTZ, Nelahozeves (margarine producer) - 190 m3 of solid fats within 5 months


Treatment of waste water with high component of surfactants:


Odor Abatement Projects :
Maturing biological ponds at WWTP JINDŘICHUV HRADEC
effluent recipient streams of WWTP BENEŠOV
waste water collector system of textile manufacturer JITKA, Jindřichuv Hradec
organically overloaded KRŇÁK lake, Zbraslav.


Bioremediation of groundwater and saturated/unsaturated zone "in-situ":
Bus company depots in SOKOLOV
Bus company depots in JIHLAVA


Revitalization of surface water reservoirs:
KRŃÁK lake in Zbraslav (odor elimination, water quality improvement, enhancement of mineralization processes in the lake sediment) by biotechnology application


Bioremediation of solid waste, sludge and soil polluted by BETX and PAH oil products:
Over 200.000 t of the above waste for hundreds of customers in 6 years.


Bioremediation of industrial offgas contaminated by VOC:
FATRA, a.s. Napajedla


Case Studies


Fat Waste Water Lagoon Bioremediation
Biological Treatment of Oil Polluted Cooling Water
ALPHA Biocatalyst Introduction into Feeding

UNILEVER-Van Den Bergh, PTZ, Inc., Nelahozeves, CZ
Fat Waste Water Lagoon Bioremediation

Initial status:
Waste water lagoon (no effluent, closed system) built in 1991 designed to last 15 years to store various types of waste waters rich in residual mostly vegetable fats, byproducts of margarine, cosmetic goods production such as fatty acids etc. plus sludge from biological WWTP and adsorbing clay. Due to failure of designing engineers to take into consideration properties of the stored waste (no evaporation of water) and annual rainfall levels in the area, the lagoon was filled within 3 years of operation. While in once third of the lagoon sediments (mostly WWTP sludge and absorbing clays) reached surface of water making about 1 meter thick layer of sediments, there were, according to our own hydrologic survey, almost no sediments on the bottom of the remaining two thirds of approx. 12.000 m3 lagoon.

The whole 4.900 m2 surface of water were covered by 3 - 10 centimeters thick monolithic layer of floating light to dark brown fats. Isolated location without power supply, water source and space/conditions for utilization of more sophisticated equipment/technology, further properties of the floating fats prohibiting mechanical manipulation with the waste, costs of the only other technology to liquidate the fat (incineration-if somebody finds how to load and package the fat) forced the customer to go for bioremediation "on-site" after on site consultations with UNILEVER´s U.K. and Dutch experts.

Initial Water quality [mg/l]:
CODCr = 8.922
TPH = 920
N-NH4 = 21,2
chlorides=1.847 sulphides=324
Ptotal = 14,7

High mineralization [caused mostly by NaCl] of 6.173,23. pH=5,3.

Water accounted for about 82% of the total volume of lagoon.
Wastes deposited to the lagoon in 1994 alone [in metric tons]:

fatty acids=209,5
fats from fat separators=114,9
residues of oil
fat and paraffin emulsions=54,5
sludge from production of margarine=8,3
sludge from soap production=226
waste from cosmetic goods production=4,3
WWTP sludge =438

Total volume of waste deposited into the lagoon in 1994 = 539
Wastes deposited to the lagoon in 04-11/1995 [in metric tons]:

fatty acids=88
residues of oil, fat and paraffin emulsions=3
used adsorbing clay=413,4
sludge from soap production=82
WWTP sludge =366

Total volume of waste deposited in the lagoon [calculation] = 2.729 m3.

One dia 1.000 mm CGU (Catalyst generating Unit) was installed in the UNILEVER´s production facility in Nelahozeves supplying CAT I. into 10 m3 storage tank, from which the CAT I. was delivered by a cistern tank three times a week into the lagoon. Saturation of the lagoon started April 1, 1995 and was finished November 30, 1995. ABES supplied to the lagoon total of 860 m3 of CAT I. increasing hereby water lever by 18-20 cm.
First application of ALPHA MICROX microbial product was done June 1, 1995 (about 100 kg) by a water cannon of a firefighting vehicle onto the surface of floating fat. ABES conducted its monitoring of processes from a raft made from steel drums. Monitoring [pH, DO, water lever, sediment depth, odor, sampling of water - N, P, K, microbial population] , conducted once a week, was quite dangerous, falling over into the lagoon meant sure death (average depth 3,1 meter). Even though "windows" manually created for each water sampling and fat layer thickness monitoring kept almost instantly closing, DO measuring proved DO content of plus minus 1,0 mg/l in the water under the fat layer. Any other aeration source and means were not available. Shortly after outside air temperature reached about 15 0C ochre patches of about 1-2meter in diameter appeared place to place on the surface of still solid fat, especially by the banks of the lagoon.
However, even though nutrient component in the water was initially adequate, and later kept adequate by supply of N,P,K fertilizers, no significant reduction in thickness of fat layer was observed. Microbial counts of 107/ml started to decline after about one month of biodegradation. First response was to check nutrient component. It was O.K. by ABES judgment. After several weeks of consultations with top Czech microbiologists we established our mistake. We failed to give proper attention to pH development. Thereafter (in the first week of August, 1995) followed applications of ammonia water - first, surface application of 5 m3 of 29% ammonia water form plastic barrels, second - 50 metric tons of the same, injected by a discharge pump of a mobile tank under the surface of fat in 12 different injection points. The effects were close to phenomenal. Intensive gas production though pores in the fat, soon open and not closing "windows" in the fat layer appeared and remained opened. Ammonia helped to dissolve fat into water making it easier for the bacteria to metabolize it. The process of dissolving of fat was clearly visible on the edges of "windows". Microbes turned mad - within a month thickness of fat decreased to 2 - 4 centimeters, on September 30, 1995 down to 0,5-1 cm.
Thereafter, at the request of the customer, ABES created moving plastic boom (about 50 m long across the lagoon) anchored to a steel rope and held in a vertical position by plastic bags filled by sand and part by part pushed the remaining fat layer to one corner of the lagoon. This boom collapsed under the pressure of depressed fat, so that new, second boom was built to finish the job. The result was, that 65% of the surface of lagoon was free of fat, clean water surface open for evaporation. We thereafter constructed permanent boom from plastic segments anchored by concrete blocks put on bottom of the lagoon. One of the major challenges and problems we faced in summer was, that fat was solid at night and till about 10 A.M., thereafter sun changed it into oil closing all windows and access to air oxygen. Nothing could be done about it. Second, customer kept dumping new fat waters and other wastes in course of bioremediation since he has had no alternative option. Yet, the following results speak for itself. If one takes into consideration that the biodegradation process started full power only in August, within 3 months Alpha Microx biodegraded approx. 190 m3 of the messy shit which is - 114% of the initial volume of fat accumulated in the lagoon at the time of initial hydrologic survey in November, 1994. - 67% of the overall fat accumulated in the lagoon till the end of bioremediation, e.i. till November 30 ,1995. Water quality sampling even the final proved negligible concentration of fat in the water and following facts >
- reduction of cations Na+ , K+ , Mg2+ , Ca2+ - probably through rainfall dilution
- due to ammonia water application > increase of NH4+, NO3- , pH of 8,4 in the fat/water contact zone
- bacterial activity reduced HPO42-, total P, increased content of HCO3 - and CO32- by release of CO2 in course of biodegradation
Final Water quality {mg.l-1}:

CODCr = 7.140
N-NH4 = 569
Ptotal = 1,52

As climate is concerned, it was short bioremediation season - 6-9/1995, but even in hot periods no odor whatsoever appeared on site. Nevertheless, Czech managers of our customer declined our offer to finish the job in 1996 saying they are very satisfied, however, wishing to save money.

KAVALIER GLASSWORKS, Inc., Sázava, the Czech Republic
Continuous Biological Treatment of Oil and Grease Polluted Cooling Water

For the treatment of oil contaminated water in a closed recycling systems of cooling water in KAVALIER ABES modified and reconstructed existing 3-chamber underground accumulation tank. The first two chambers were changed into sludge activation tanks equipped by microbubble aeration segments. Further, ABES installed large surface biomass carriers (8 m3, usually used in cooling towers) in the second chamber . Submersible suction pump installed in the second chamber arranged for return of activated sludge in the first chamber. The overall volume of activated sludge tank (both chambers) is 40 m3, Qmax was 35 m3, TPHmax = 50 mg.l-1. Theoretical TPH removal efficiency is 90%. Additionally, operation of pumps (interval of pumping) in the third chamber (delivering water to cooling towers and concrete tank under the cooling towers - further referred to as CT tank) has been changed to prevent any oil to leave first two chambers. ABES installed and put into operation (in a building adjacent to the bioreactor) 3 CGUs delivering total of 75 liters of CAT I. an hour into the first chamber of the bioreactor. The 4 month long trial operation began on January 16, 1995.

Sample were taken and analyzed by an independent certified lab at least once a week in two measuring points :
- intake into the first chamber of bioreactor (in enclosed table - PAJ) influent quality
- exit from pumping tank situated in the circuit behind CT tank and hydrocyclones.

Results of the trial operation of bioreactor :
Bioreactor volume : 40 m3
Qmax = 35 m3.h-1
TPHmax = 50 mg.l-1
Required TPH content limit ... 1,0 mg.l-1 or lower
Volume of daily added fresh water into the system QD = 66 m3/day
Volume of pumping tank is 600 m3
Average TPH influent concentration (accidents excluded) = 14,7 g. m-3
Total content of TPH in the system ................................ 8.820 grams
TPH removed by evaporation and loss in the circuit ..... 970,2 grams
Final average TPH concentration arrived to by mass balance calculation = 13,08 g.m-3
Average BOD removal efficiency EBOD = 73,8 %

Slower than expected fine-tuning of the systems was caused by initial inadequate supply of nutrient into the system and further extremely high concentration of TPH in the system in the first week of the trial (estimate= about 5 m3 of raw floating oil product) and during an accident of shock loading of about 300 liters of oil on February 12, 1995). Beginning march 22, 1995 when ABES stopped using ammonia nitrate and replaced it by urea, which is better accessible by the microorganism, significant improvement in treatment has been monitored. Bioreactor managed to return the system back to normal situation within 7 days after the a/m February 12, 1995 accident). Bioreactor operation has been stable even though variation of influent TPH concentration was 86,1%. Accident and constant pressure of ABES made the customer take measures in its operation that significantly reduced average TPH concentration and frequency of accidents. Regardless efficiently of TPH removal exceeds at all times 92% regardless the loading. In fact, the higher loading the higher efficiency is documented. Current price of treatment (incl. write-offs from installed equipment) amounts to CZK 8,00 or 0,30 $ per m3 of treated water.

Final remarks: the system works to customer satisfaction, he saves on fresh water, the system transforms dangerous waste into usual waste water treatment sludge producing hereby savings {in past they destroyed the removed oil by incineration in a utility}. Effluent TPH concentration is constantly between 0,1- 0,3 mg.l-1. In course of November 1995 accident, when during a fire in one of production facilities, about 5 m3 of oil product splashed into the bioreactor, the system managed to destroy all of the pollutant within 3 days securing no harm to production at all. Further, the glassworks in question is dynamically developing company that is increasing its production. While the system was designed to treat maximum 35 m3 per hour, now, for months the average hydraulic loading of the system is between 50 - 60 m3 per hour. Even though bioreactor retention time has been reduced by this change to 40 -48 minutes - the effluent quality has not deteriorated.

The beauty of this kind of projects is
- most companies dealing with oil products in their waste waters already do have accumulation tanks, sinks, basins where they by primitive means try to separate oil from water (mechanical oil separators, sand filters etc.)
- you do not require any costly and site consuming construction activities
- if you arrange for aeration and adequate nutrient supply - IT WORKS LIKE HELL
- the system is simple requires few minutes of attention a day, no waste water expert, mostly no additional energy consumption, make things easier and cheaper in waste management and removal(dumping)
- it is cheaper both in terms of investment and operational costs that usual chemical treatment methods, capacity of the system can be significantly stretched, and once customer finds how it works - he becomes addict.

Evaluation of KAVALIER GLASSWORKS Project in Sázava - clarification

Flow pattern in the treatment of oil contaminated water in a closed recycling systems of cooling water in KAVALIER is as follows : cooling water gets polluted in contact with an equipment cutting melted glass. After the water cools the glass products it flows directly into ALPHA bioreactor. Effluent from bioreactor flows into one hydrocyclone wherein heavier solids are separated. Effluent quality (efficiency of bioreactor) is sampled and measured behind this hydrocyclone. Thereafter cooling water flows into cooling microtowers and CT-tank under the cooling towers. Cooling towers receive cooling water from 3 individual cooling water circuits, but only one, the treated one, deals with problems of oil/grease/lubricant contamination. From CT-tank water is pumped to underground tank of pumping station that delivers cooling water back to all three independent cooling water circuits (places of utilization). Important fact is, that provided results are not affected by dilution, since measuring of bioreactor effect is conducted in a measuring point prior to a place where cooling waters of all three circuits meet in cooling towers.

Sludge production - activated sludge settles in all segments of the system - from bioreactor to pumping station tank. We have no presentable and reliable data on sludge production, since most tanks have limited and difficult access. Operator cleans the system from accumulated sludge time-to-time by suction pump and delivers it to a dump. We expect that such an operation will have to be done once in 2-3 years in the part of the system behind bioreactor. Bioreactor is cleaned by suction pump once in 6 months - on average they remove approx. 6 m3 of sludge/water mix of 1% or 10 g/l SS content.

Evaluation of a Test Conducted in Pomnenice Pig Farm Enhancement of Average Daily Gain in Weight and Improvement of Health Status, Reduction of Mortality of Animals through ALPHA Biocatalyst Introduction into Feeding

56 pieces of randomly selected animals were divided into 7 groups of 8 animals each. Further, 3 of these groups were marked as test groups, remaining groups served as control groups. Test groups (further abbreviated TG) have been subject to application of Alpha BioCatalyst in various dilution as follows :
TG-2 - 10 liters of CAT-1 in 50 liters of potable water
TG-3 - 5 liters of CAT-1 in 50 liters of potable water
TG-1 - 25 liters of CAT-1 in 50 liters of potable water

All groups were fed with the same quality and quantity of feeding three times a day (wet feeding). The feeding was premixed in Brunnthaler mixing divide and supplied to animals pneumatically. The feeding process is controlled by a computer, that is the feed rate is automatic as per number of animals in the lot and their current weight. The rate between feeding mixture and water was 1 : 3.

In course of the first weighting on January 11, 1995 ( 5 weeks into trial) no difference between TG and control groups (further abbreviated CG) was established. ABES suspected that very low temperature in stable (about 5 Degrees Centigrade) and low temperature (8-10 Degrees of Centigrade) of water in off-site CDU used as a source of Alpha BioCatalyst may be responsible for failure. Another off-site source of Alpha BioCatalyst started to be utilized (operating with water of 25 Degrees of Centigrade).

The second weighting was conducted on February 6, 1995. Again, no significant difference between TGs and CGs. In spite of unexpected failure to increase ADG of animals in this first part of the test, discussed further, very significant improvement was noticed in effects of Alpha BioCatalyst on health status and mortality. CG has significantly higher mortality - 4 animals between Jan. 23 - Feb. 17, 1995). ABES suspected the animals are overdosed by too high of a concentration of Alpha BioCatalyst, hence, the concentration was reduced by 50 per cent, compared to the original concentration. Further, the animals received Alpha BioCatalyst in following concentrations :
TG-3 - 2.5 liters of CAT-1 in 50 liters of potable water
TG-2 - 5 liters of CAT-1 in 50 liters of potable water
TG-1 - 15 liters of CAT-1 in 50 liters of potable water

The third last weighting was conducted on April 10, 1995 during shipping of both the TGs and CGs into slaughter house. Average daily gains were established as follows :
TG-3 - 0.587 kg per animal per day in net weight
TG-2 - 0.552 kg per animal per day in net weight
TG-1 - 0.543 kg per animal per day in net weight

Average daily gain of all three test groups was 0,561 kg per animal per day in net weight.

Average daily gain of all four test groups was 0,497 kg per animal per day in net weight.

Average difference between ADG of TGs and CGs was 12.9 per cent. The minimum difference was established with TG-1 - 9,25 per cent, the maximum difference with TG-3 - 18.1 per cent.

The test in POMNENICE FARM proved or suggested the following :
1) The failure to achieve positive results in ADG during the first phase of the test can hardly be attributed to inadequate quality of Alpha BioCatalyst produced by the "cold" CDU used as off-site source initially. Rather, too high concentration of Alpha BioCatalyst was applied. The positive results in ADG were arrived to only in course of last 8 weeks.
2) Difference between ADG of TGs and CGs is beyond framework f a statistical error.
3) Alpha BioCatalyst has major influence on improved utilization/feed conversion of feeding. The lowest concentration applied showed the best results in ADG improvement. Finding of the importance of the necessity to apply low concentration of Alpha BioCatalyst is one of significant positive results of the test to ABES.
4) The test was heavily influence by extremely bad climatic conditions - outside temperatures ranged from - 10 to - 20 C for about 9 weeks of the test. Inside temperature in the stable was very low ( 5 - 7 C) in the same period. It is highly likely, that test results might have been even better if the test was conducted not under such extremely adverse climatic conditions. The low stable temperature has probably slowed animal metabolism.
5) Test proved capability of Alpha BioCatalyst to reduce, or even eliminate illness and mortality of the animals treated, it is likely that it may reduce medicine consumption and its costs. In TG no animal died, no illness was established, in CGs 4 animals died and several ill animals had to be veterinary treated .
6) The selection of animals for the was far from being optimal. Animals of both TGs and CGs were brought in for the test from 3 different locations and the piglets had, therefore, different growing conditions influenced by different quality of care of type of feeding.
7) POMNENICE Test proved that even under local (Czech) climatic conditions results achieved in similar test in RSA by Alpha BioTech c.c. may be achieved to some extent too.
8) Theoretical economic benefits :
possible reduction of resident/feeding time (turnover of herd) by 15 and more per cent, that is with TG-3 by 20,1 days. possibility to reduce consumption of feeding by 15 and more per cent based on results of TG-3 and 600 pieces of treated animals the net benefit translates into increase of sales by CZK 342,000.00 or US Dollars 10,500.00.

AlphaBio EcoService, s.r.o.

Ing. Josef Šimíček, Mgr. Vladimír Skoupý
Postal address: Vlastina 23, 161 00 Praha - 6
tel. +420-2-96 40 08 03, fax. +420-2-33 31 30 86
E-mail: abes@wo.cz
RNDr. Vladimír Suk
Postal address: Poděbradova 32, 701 00 Moravská Ostrava
tel./fax. +420-69-612 51 68
E-mail: suk.abes@worldonline.cz
Ing. Jaroslav Kraus
tel. +420-182-92 371

Autor: Martin Kuciel Datum poslední úpravy: 22.10.2000